Harvard University Paper Chromatography Lab Report this is a lab report for experiment called Paper Chromatography the data is attached on the files. Also

Harvard University Paper Chromatography Lab Report this is a lab report for experiment called Paper Chromatography

the data is attached on the files. Also,The experiment is attached on the file as well.

Introduction: This should be one or two sentences that explain(s) what you plan to do during the laboratory experiment.

Procedure: . This is to be in your own words, not copied from the laboratory manual. Do not include laboratory observations in the procedure section. This section is typically written in the past tense third person. Do not write outlines, numbered lists, or bulleted lists. This section must be written in paragraph form

Please do NOT copy the manual

400 words exp04.qxd
11/15/13
9:33 AM
Page 83
Experiment 4 Report Sheet
Paper Chromatography
Date __________ Lab Sec. ______ Name ____________________________________________ Desk No. __________
C.4. Analysis of the Chromatogram for Individual Ions
Distance of eluent front from the origin, Deluent: __________ mm
Color
(original)
Color
(with NH3)
Color
(with spot solution)
Distance (mm)
Traveled, Dion
Rf
Mn2+
______________
______________
______________
______________
______________
Fe3+
______________
______________
______________
______________
______________
Co2+
______________
______________
______________
______________
______________
Ni2+
______________
______________
______________
______________
______________
Cu2+
______________
______________
______________
______________
______________
Instructor’s approval of chromatogram __________________________________________________________________
Show your calculations for Rf.
Experiment 4
83
exp04.qxd
11/15/13
9:33 AM
Page 84
C.5. Analysis of the Chromatogram of Unknown Mixtures
Unknown number(s)
U1=_________
Band 1
Unknown
Distance
(mm)
Traveled, Dion
U2=_________
Band 2
Rf
Distance
(mm)
Traveled, Dion
U3=_________
Band 3
Rf
Distance
(mm)
Traveled, Dion
Band 4
Rf
Distance
(mm)
Traveled, Dion Rf
U1
__________ ________
__________ ________
__________ _________
__________ _______
U2
__________ ________
__________ ________
__________ _________
__________ _______
U3
__________ ________
__________ ________
__________ _________
__________ _______
Show your calculations for Rf.
Cations present in U1
________
________
________
________
Cations present in U2
________
________
________
________
Cations present in U3
________
________
________
________
Laboratory Questions
Circle the questions that have been assigned.
1. Part A.1 and Part B.3.
a. Why is it important to keep the developing chamber covered with plastic wrap during the development of the
chromatogram?
b. The developing chamber is not covered with plastic wrap during the development of the chromatogram. Will the
Dion value for the cation be greater than or less than the actual value as a result of this technique error? Explain.
2. Part A.4. Explain how the appearance of the chromatogram might change if the chromatographic paper were initially
wet with water or had come in contact with the skin.
3. Part A.4. Why was a pencil used to mark the chromatogram and not a ballpoint or ink pen?
4. Part B.1. Explain why the cation samples are repeatedly spotted and dried on the chromatographic paper.
5. Part B.3. The eluent is to be below the 1.5-cm line on the chromatographic paper. Describe the expected observation if
the eluent were above the 1.5-cm line.
6. Part C.2. Explain why the center of the band is used to calculate the Rf value for a cation rather than the leading edge
of the band.
7. Suppose two cations have the same Rf value. How might you resolve their presence in a mixture using paper
chromatography?
84
Paper Chromatography
C.5. Analysis of the Chromatogram of Unknown Mixtures
Unknown number(s) U1=
U2-
Band 1
Band 2
U3-
Band 4
Distance
(mm)
Unknown Traveled, D
Distance
(mm)
Traveled, D
Band 3
Distance
(mm)
Traveled,
DR.
Distance
(mm)
Traveled, D… R
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Experiment 4 Report Sheet
Paper Chromatography
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Desk No.
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Sec. Name
C4. Analysis of the Chromatogram for Individual lons
Distance of cluent front from the origin, De
mm
Color
Color
Color
(original) (with NH) (with spot solution)
.com
Distance (mm)
Traveled, D..
RE
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to
the
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Mo
avel
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write
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yellow
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ayer
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Low 0.40
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Instructor’s approval of chromatogram
the
iron oronum
60
Show your calculations for R
/
Tona
Festa
con
O
27
– 0.40
100
2-4
??2)
= 45 = 0.45
Experiment 4
83
700

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